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      2. 不同脂肪含量飼料對吉富羅非魚魚種生長性能、脂蛋白脂酶活性及其基因表達的影響
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        江蘇省省屬高校自然科學基礎研究項目(08KJD240003)和江蘇省第四期“333高層次人才培養工程”第三層次培養對象資助項目


        Effects of dietary lipid levels on growth performance,activity and gene expression of lipoprotein lipase in GIFT tilapia (Oreochromis niloticus) juvenile
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          摘要:

          試驗選用均質量(2.63±0.16 g)的吉富羅非魚魚種315尾,隨機分成3組,每組3個重復,每個重復35尾,分別飼喂3.7%、7.7%和16.6%的低、中、高脂肪水平的等氮飼料,探討不同脂肪含量飼料對吉富羅非魚魚種生長、肝體指數、脂肪沉積、脂蛋白脂酶(lipoprotein lipase,LPL)活性及其基因表達的影響,并初步探討LPL基因表達與脂肪沉積的關聯規律,試驗周期90 d。結果顯示:(1)飼喂高脂飼料的試驗魚肝體指數、臟體指數顯著升高,肥滿度無顯著變化,肝臟與肌肉脂肪含量顯著升高; (2)飼喂高脂肪飼料試驗魚的增重率顯著下降、飼料系數下降顯著;(3)LPL基因在吉富羅非魚魚種肝臟和肌肉中均有表達,但肝臟中LPL mRNA表達豐度顯著高于肌肉;(4)在再投喂48 h內,在第6小時時,高脂肪試驗組魚肝臟LPL活性顯著高于中、低脂肪試驗組,但隨后顯著降低,顯著低于3.7%試驗組;7.7%和3.7%試驗組魚肝臟LPL活性呈現先升高后下降的趨勢;(5)飼喂高脂飼料的試驗魚肝臟中LPL mRNA表達豐度最高,顯著高于3.7% 低脂試驗組;(6)吉富羅非魚肝體指數、肝臟和肌肉脂肪含量與肝臟LPL表達具有顯著的線性正相關。結果表明,LPL mRNA在吉富羅非魚肝臟和肌肉中表達具有組織特異性;高脂飼料顯著抑制了吉富羅非魚魚種增重;高脂飼料顯著促進了吉富羅非魚肝臟LPL的分泌,誘導了其肝臟中LPL基因表達,促進肝臟與肌肉脂肪沉積; LPL基因參與調節吉富羅非魚脂肪沉積。

          Abstract:

          A 90-day study was conducted to investigate effects of dietary lipid levels on performance,fat deposition,activity and expression of lipoprotein lipase (LPL) in GIFT tilapia (Oreochromis niloticus) juvenile,and to explore the correlation between LPL gene expression and fat deposition.315 GIFT tilapia juveniles(average weight 2.63±0.16 g) were randomly divided into three groups with three replications,and fed with isonitrogenous diets with different lipid levels of 3.7% (low-lipid group),7.7%(middle-lipid group)and 16.6% (high-lipid group) by supplementing with 2%,6% and 15% fish oil as lipid source,respectively.The results showed:(1) the hepatosomatic index (HSI),viscerosomatic index (VSI) and lipid contents in liver and muscle increased with increasing dietary lipid level(P<0.05),but there was a trends for condition factor (CF) with first increased then decreased (P>0.05); (2)with increasing dietary lipid levels, the weight gain (WG) first increased, then decreased, and feed conversion ratio (FCR) decreased(P<0.05); (3)LPL gene was expressed in liver and muscle of GIFT tilapia,while the expression level in liver was significantly higher than that in muscle (P<0.05); (4) during the 48 h post-refeeding period,liver LPL activity in high-lipid group was significantly higher than that in low-lipid and middle-lipid group at 6 h,but significantly lower than that in low-lipid group at the 12 h,24 h and 48 h.There was a trend in low-lipid and middle-lipid group that liver LPL activity increased first and then decreased; (5) the expression of LPL was significantly higher in high-lipid group than that in low-lipid group (P<0.05); (6) there were a significant linear positive correlation between HSI,lipid contents in liver and muscle,and liver LPL expression in GIFT tilapia juvenile.The results indicated that LPL expression in GIFT tilapia was in a tissue specific pattern and liver was the main organ for secretion and expression of LPL; high dietary lipid level could inhibit growth of GIFT tilapia juvenile,decrease feed conversion ratio,induce the expression of LPL in liver,and promote fat deposition in liver and muscle; liver LPL expression was involved in fat deposition of GIFT tilapia juvenile.

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        王愛民,楊文平,於葉兵,呂富,吳文靜,封功能,王資生.不同脂肪含量飼料對吉富羅非魚魚種生長性能、脂蛋白脂酶活性及其基因表達的影響[J].華中農業大學學報,2014,33(2):96-102

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        • 收稿日期:2012-12-31
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