國家重點研發計劃項目（2018YFD0201006）； 國家自然科學基金項目（31670243）； 華中農業大學科技創新基金項目（2662017PY052,2662017PY121）
從大豆根際及根瘤中分離獲得 2株具有較高解磷能力的細菌PSB-1和HZP1，通過Salkowski比色法和鉬銻鈧比色法,對這 2個菌株產生IAA（indole-3-acetic acid,IAA）的能力和溶解無機磷的能力進行了測定，同時對紫云英和大豆進行接種，并對田間栽培的大豆進行了菌劑接種試驗。結果顯示：PSB-1及HZP1均具有解磷及產IAA的特性;單接種解磷菌HZP1或PSB-1均有促進紫云英和大豆生長的作用；相比于單接種根瘤菌，將這2株解磷細菌分別與根瘤菌混合接種能進一步提高大豆和紫云英的地上生物量、根瘤鮮質量和根瘤數。田間試驗結果顯示，單獨接種根瘤菌劑或解磷菌劑都有一定的增產效果，但雙接種根瘤菌劑和解磷菌劑的增產效果不明顯。
Two phosphate solubilizing bacterial strains,PSB-1 and HZP1,were isolated form soybean nodules and rhizosphere soil. The ability of these two strains to produce IAA (indole-3-acetic acid IAA) and the ability to dissolve inorganic phosphorus were measured by Salkowski colorimetry and molybdenum antimony-phosphonium colorimetry. Soybean and Astragalus sinensis were co-inoculated. Results showed that both two phosphate solubilizing strain produced IAA. The legume growth-promoting effects of the phosphate solubilizing strains and rhizobium inoculum were investigated in unsterilized soil and soybean planting field. Inoculation with HZP1 or PSB-1 promoted the growth of Astragalus sinensis and soybean. Co-inoculation of phosphorus-solubilizing strains with rhizobium further increased the shoot biomass,fresh weight of nodules and the number of nodules of soybean and Astragalus sinensis. The results of field tests showed that inoculation with rhizobium or phosphate dissolving agents alone had a certain effect of increasing yield but double inoculation with rhizobium and phosphate dissolving bacteria did not increase the yield significantly.