• <mark id="l0x4h"></mark>

      <u id="l0x4h"></u>
        <ins id="l0x4h"></ins>

      1. <bdo id="l0x4h"></bdo>
      2. 混酶水解法制備的鰱魚皮多肽的抗氧化活性
        DOI:
        作者:
        作者單位:

        作者簡介:

        通訊作者:

        中圖分類號:

        基金項目:

        現代農業產業技術體系專項(CARS-45-28);湖北省技術創新專項重大項目(2017ABA141)


        Antioxidant activity of polypeptides from silver carp skin prepared by mixed enzymatic hydrolysis
        Author:
        Affiliation:

        Fund Project:

        • 摘要
        • |
        • 圖/表
        • |
        • 訪問統計
        • |
        • 參考文獻
        • |
        • 相似文獻
        • |
        • 引證文獻
        • |
        • 資源附件
        • |
        • 文章評論
          摘要:

          以鰱魚皮為原料,采用混酶水解法制備鰱魚皮抗氧化肽,經超濾膜分離得到SP1(>6 ku)、SP2(3~6 ku)、SP3(<3 ku)3種組分,研究不同分子質量組分的體外抗氧化能力及SP1和SP3組分的氨基酸組成,并選出體外抗氧化能力最強的SP3組分,進行體內抗氧化活性評價。結果表明:3種組分的羥自由基清除率、超氧陰離子自由基清除率和還原能力從高到低的順序均為SP3>SP2>SP1,而銅離子螯合率無顯著差異;氨基酸分析結果顯示SP3組分中疏水性氨基酸所占比例較高;體內抗氧化試驗顯示:相較于損傷模型組,灌服SP3組分的小鼠肝臟和脾臟指數明顯增大,且血清和肝臟中的超氧化物歧化酶(SOD)、谷胱甘肽過氧化物酶(GSH-Px)和過氧化氫酶(CAT)的酶活力明顯提高,丙二醛(MDA)含量明顯降低。綜上可知,SP3組分在體內外均具有較好的抗氧化活性。

          Abstract:

          The polypeptides were prepared from silver carp skin by the method of mixed enzymatic hydrolysis,and divided into SP1(>6 ku),SP2(3-6 ku) and SP3(<3 ku) by ultrafiltration. The antioxidant capacity in vitro of three polypeptides and the amino acid composition of SP1 and SP3 were analyzed. It was suggested that SP3 has the strongest antioxidant capacity in vitro. Subsequently,the antioxidant capacity in vivo of SP3 was measured. The results showed that the order of hydroxyl radical scavenging rate,superoxide anion radical scavenging rate and reducing ability in the three polypeptides was SP3>SP2>SP1,but there was no significant difference in chelating rate of copper ions. Analysis of amino acid composition showed that the percentage of hydrophobic amino acid in SP3 was significantly higher than SP1. Besides, compared with the injured group,SP3 group had higher indexes of liver and spleen. Furthermore,SP3 significantly increased the activities of SOD,GSH-Px and CAT and decreased the content of MDA in serum and liver. In conclusion,SP3 has better antioxidant activity than SP1 and SP2.

          參考文獻
          相似文獻
          引證文獻
        引用本文

        武潤琳,劉建偉,熊善柏,胡楊,尤娟,劉茹.混酶水解法制備的鰱魚皮多肽的抗氧化活性[J].華中農業大學學報,2020,39(4):114-120

        復制
        分享
        文章指標
        • 點擊次數:
        • 下載次數:
        • HTML閱讀次數:
        • 引用次數:
        歷史
        • 收稿日期:2020-03-12
        • 最后修改日期:
        • 錄用日期:
        • 在線發布日期: 2020-07-30
        • 出版日期:
        牌九游戏