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      2. 3種芽孢桿菌菌劑對黃瓜枯萎病的防效及其作用機制初探
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        國家自然科學基金項目(41601257);中國博士后科學基金項目(2015M580653);農業微生物學國家重點實驗室開放課題(AMLKF201708)


        Control effect of three Bacillus strains on cucumberfusarium wilt and its mechanism
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          摘要:

          以含有黃瓜枯萎病病原菌(尖孢鐮刀菌)的病土為材料,采用盆栽試驗研究巨大芽孢桿菌B213、多粘類芽孢桿菌B207和枯草芽孢桿菌B204單獨或聯合施用對黃瓜枯萎病的防效,并對其作用機制進行初步分析。結果顯示:3種芽孢桿菌單獨或組合施用后均可有效抑制黃瓜枯萎病的發生,并顯著增加黃瓜植株的生物量,且三菌劑聯用>雙菌劑聯用>單菌劑施用。在黃瓜幼苗移栽后35 d,與對照組相比,三菌劑聯用組黃瓜枯萎病的發病率降低了79.99%;植株干質量提高了6.92倍;植株抗性酶如苯丙氨酸解氨酶、〖JP2〗多酚氧化酶、過氧化物酶活性分別提高了60.02%、28.90%和108.60%;根際土壤尖孢鐮刀菌數量下降了2個數量級。該防病促生效果可能與這3種芽孢桿菌對病原菌的拮抗作用、溶磷能力以及吲哚乙酸、蛋白酶、幾丁質酶和嗜鐵素的分泌能力有關。

          Abstract:

          The pot experiment with the soil containing pathogens of Fusarium oxysporum f. sp. was conducted to study the effects of Bacillus megaterium B213,Bacillus polymyxa B207,and Bacillus subtilis B204 alone or in combination on cucumber fusarium wilt and its mechanism. The results showed that the three microbial agents alone or in combination effectively inhibited the occurrence of cucumber fusarium wilt and significantly increased the biomass of cucumber. The effect of the two microbial agents in combination was better than that of single microbial agent. The effect of the three microbial agents in combination was better than that of the two microbial agents. Compared with the control group,the incidence of cucumber fusarium wilt in the three-agent combination group when cucumber seedlings were transplanted 35 days ago reduced by 79.99%; the dry weight of cucumber plants increased by 6.92 times; the activities of plant resistance enzymes including acid lyase,polyphenol oxidase,and peroxidase increased by 60.02%,28.90%,and 108.60% respectively; the number of rhizosphere soil Fusarium oxysporum decreased by 2 orders of magnitude. The effect of disease prevention and growth promoting may be related to the antagonism on the pathogenic bacteria,the ability to dissolve phosphorus,and the secretory ability of indoleacetic acid,protease,chitinase and ferrophilin of these three Bacillus species.

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        季倩茹,陳靜,胡遠亮,梁運祥,胡詠梅.3種芽孢桿菌菌劑對黃瓜枯萎病的防效及其作用機制初探[J].華中農業大學學報,2020,39(5):101-107

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        • 收稿日期:2019-12-16
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        • 在線發布日期: 2020-10-05
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