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      2. 黃單胞菌分泌蛋白的理化性質及特征分析
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        國家自然科學基金項目(31960314);云南省應用基礎研究計劃項目(2018FG001-028);云南省研發經費投入補助項目(2016YB462&2017YB236)


        Analyzing physicochemical properties and characteristics of Xanthomonas campestris secretary protein
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          摘要:

          基于全基因組序列已經公布的黃單胞菌Xanthomonas campestris B100、Xanthomonas campestris pv. campestris 8004、Xanthomonas campestris CN14中分泌蛋白序列為基礎數據,采用Protscale、SMART、TargetP 2.0 Server等生物信息學分析軟件對上述分泌蛋白展開理化性質、保守結構域以及轉運肽等分析,結果顯示:黃單胞菌中分泌蛋白理論等電點與氨基酸長度之間無相關關系, 44.73%的蛋白為不穩定蛋白, 83.21%的蛋白親水性小于0,屬于親水性蛋白。同時,在每種黃單胞菌的分泌蛋白中,具有明顯保守結構域的蛋白數量平均為12個,并且所有分泌蛋白均具有信號肽,轉運肽預測可能性分布也較為平均。

          Abstract:

          Xanthomonas campestris belong to Gram-negative bacteria,which mainly damage cruciferous vegetables and economic forests including walnuts and mangosteens,causing great economic losses to agricultural and forestry crops and seriously harming the healthy development of the agricultural and forestry industry. Secreted proteins play an important role in the pathogenic process of plant pathogenic fungi,bacteria,and oomycetes. There is no reports on the physicochemical properties and characteristics of secretary proteins of Xanthomonas. This study uses the secretary protein sequences of X. campestris B100, X. campestris pv. campestris str.8004,and X. campestris CN14 from the published genome-wide sequence as the basic data,and uses Protscale,SMART,TargetP 2.0 Server,and other bioinformatics software to analyze the physicochemical properties,conserved domains,and transit peptides of the secretary proteins above-mentioned. The results showed that there was no obvious rule between the theoretical isoelectric point of secretary proteins and the length of amino acids in X. campestris. An average 44.73% of the protein is unstable protein. An average of 83.21% of the protein has a total average hydrophilicity of less than 0,which is a hydrophilic protein. The number of proteins with obvious conserved domains in each secretary protein of Xanthomonas is 12 on average. All secretary proteins have signal peptides. The distribution of transit peptide prediction possibilities is relatively even.

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        覃悅,祝友朋,韓長志.黃單胞菌分泌蛋白的理化性質及特征分析[J].華中農業大學學報,2020,39(6):30-36

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        • 在線發布日期: 2020-12-01
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