Taking Nemipterus virgatus surimi as the research object,the effect of transglutaminase on the 3D printing characteristics of Nemipterus virgatus surimi was studied from three aspects:apparent viscosity and rheological properties (difficulty or ease of conveying),printing effect (appearance,molding performance),and the properties of surimi product. As the transglutaminase addition increased,the gel strength,elasticity,and water holding capacity of Nemipterus virgatus surimi gel increased firstly and then decreased. When the transglutaminase addition was 0.2%,they reached the highest value,which were increased by 70.88%,25.01%,and 9.72% compared with the control group,respectively. The relative content of immobilized water in the surimi reached the maximum when the transglutaminase was added at 0.2%,and the T23 peak ratio was 0.97. The apparent viscosity,yield stress,consistency and rheological properties of Nemipterus virgatus surimi gradually increased with increasing transglutaminase addition,while the fluidity decreased. The apparent viscosity reached the maximum at 0.4% transglutaminase,and loss modulus and loss tangent at 0.2% transglutaminase. The surface of the sample with 0.2% transglutaminase was relatively integral and matched the set model,and the continuity and adhesion between the surimi threads were relatively high. In summary,addition of 0.2% transglutaminase was the best for printing the Nemipterus virgatus surimi gel product.