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      2. 莖瘤芥BXL基因家族的全基因組鑒定及在瘤狀莖膨大過程中的表達分析
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        國家自然科學基金項目(31701928); 重慶市科技局基礎科學與前沿技術研究專項(cstc2017jcyjAX0226); 重慶市教委科學技術研究項目(KJ1712305); 長江師范學院校級科研項目(2016KYQD19和2016XJQN07)


        Genome-wide identification of BXL family genes in Brassica juncea var.tumida and their expression during swelling of stem
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          摘要:

          為深入解析BXL家族基因在莖瘤芥發育中的功能,利用生物信息學方法從莖瘤芥全基因組鑒定出16個BjuBXL,并系統分析了BjuBXL家族蛋白的理化性質、基因結構、保守基序、染色體定位和在莖膨大過程中的表達。結果表明:16個BjuBXL基因分為7類,共2個亞族,其中BjuBXL1-1~ BjuBXL1-4與擬南芥中參與細胞壁代謝和植物發育的AtBXL1親緣關系相近;7個BjuBXL定位于A亞基因組的5條染色體上,8個定位于B亞基因組的4條染色體上;熒光定量PCR分析表明BjuBXL1-1和BjuBXL1-2在莖膨大過程中均明顯上調,而且在莖的內髓區(P2~P4)表達量均顯著高于外髓區(P1),可能參與莖膨大過程中莖內髓區的細胞壁代謝。

          Abstract:

          In order to further analyze the functions of BXL family genes in the development of stem,16 genes encoding putative BXL proteins were identified in the whole genome of Brassica juncea var. tumida with bioinformatics methods. The gene structure and conserved motifs,chromosomal distribution and gene expression during the swelling of stem of BjuBXL family genes and the physicochemical properties of BjuBXL family protein were systematically analyzed. The results showed that 16 BjuBXL genes were classified into 7 categories and 2 sub-families according to the phylogenetic tree analysis,among which BjuBXL1-1,BjuBXL1-2,BjuBXL1-3 and BjuBXL1-4 are closely related to AtBXL1,which is involved in cell wall metabolism and plant development in Arabidopsis. 7 BjuBXL genes were located in 5 chromosomes of A subgenomes and 8 BjuBXL genes are located in 4 chromosomes of B subgenomes. The results of fluorescence quantitative PCR analyses showed that the transcriptional levels of BjuBXL1-1and BjuBXL1-2 were up-regulated obviously during the swelling of stem. The expression in the inner pith (P2-P4) were significantly higher than that in regions of the outer pith of swollen stem (P1),indicating that BjuBXL1-1 and BjuBXL1-2 may play an essential role in the cell wall metabolism of inner pith during the swelling of stem.

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        賴彪,陳春帆,劉春渝,朱純真,杜麗娜.莖瘤芥BXL基因家族的全基因組鑒定及在瘤狀莖膨大過程中的表達分析[J].華中農業大學學報,2020,39(6):155-163

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        • 收稿日期:2020-05-28
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        • 在線發布日期: 2020-12-01
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