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      2. 雌激素受體基因對泥鰍雌雄和倍性生長差異的表達調控作用
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        國家重點研發計劃藍色糧倉創新專項(2018YFD0900205);國家自然科學基金項目(31872559);國家大宗淡水魚類產業技術體系建設項目(CARS-46-08);中央高?;究蒲袠I務費專項(2662018PY035)


        Regulation of ER genes on growth of different sex and ploidy in Misgurnus anguillicaudatus
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          摘要:

          為探討雌激素受體基因與魚類雌雄及倍性生長差異之間的相關性,選取雌性生長快于雄性、且四倍體生長快于二倍體的泥鰍為研究對象,首先克隆泥鰍雌激素受體基因,獲得ERα(1 821 bp)、ERβ1(1 008 bp)和ERβ2(1 197 bp)的cDNA序列,氨基酸序列比對發現泥鰍ERs分別與鯉科魚類相應亞型具有較高的同源性。采用qRT-PCR檢測ER基因在二倍體泥鰍性成熟前后5個關鍵時期的表達情況及二倍體、四倍體相關組織間的差異表達。結果顯示,3個基因在雌雄個體性成熟前后腦、肌肉、肝臟和性腺組織中均存在差異表達,其中ERα 在泥鰍雄性個體腦、肌肉和性腺組織中的表達量顯著高于雌性(P<0.05),而在雌性肝臟組織中的表達量普遍高于雄性(P<0.05);ERβ1和ERβ2在精巢組織中的表達量一直處于較高水平。泥鰍倍性間的比較結果顯示,3個ER基因在泥鰍二倍體腦組織中的表達量顯著高于四倍體的表達量,且雄性的表達量均高于雌性,其中〖JP2〗ERα表達差異最為顯著。本研究結果表明,無論是針對倍性生長差異還是雌雄生長差異,ERα均表現為在生長較慢(二倍體、雄性)個體腦組織中有相對高表達,推測腦組織中ERα 基因對魚類個體生長速度具有一定抑制作用。

          Abstract:

          In order to investigate the relationship between estrogen receptor (ER) genes and growth of different sexes and ploidy in fish,loach(Misgurnus anguillicaudatus)whose female growth is faster than that of male and whose tetraploid growth is faster than diploid were selected as the research object in this study.We firstly cloned the cDNA sequences of three ER genes of loach,including ERα (1 821 bp),ERβ1 (1 008 bp) and ERβ2 (1 197 bp).Amino acid sequence alignment revealed that the loach ER genes shared higher similarity with corresponding subtypes of carps.Quantitative RT-PCR was used to detect the expression of ER genes at five growth stages of diploid loach,as well as in different tissues in diploid and tetraploid adults.The results showed that the three ER genes were differentially expressed in the brain,muscle,liver,and gonadal tissues of the male and female individuals before and after sexual maturity.Among them,the expression of ERα in the brain,muscle and gonad tissues of males was significantly higher than that of females (P<0.05),and expression in the female liver was generally higher than that in males (P<0.05).The expression levels of ERβ1 and ERβ2 in the testis were always high.Comparison results between different ploidy showed that expression of the three ER genes in the diploid brain were significantly higher than that of the tetraploids,and the expression levels of males were higher than that of females,with the expression of ERα being the most significant.The results of this study indicated that regardless of the difference between ploidy and sex growth, ERα appears to have relatively high expression in the brain of slow-growing (diploid,male) individuals.Therefore,we speculate that the ERα gene in the brain might have a certain inhibitory effect on the growth rate of fish.

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        李丹陽,羅麗飛,曹文怡,周小云,高澤霞.雌激素受體基因對泥鰍雌雄和倍性生長差異的表達調控作用[J].華中農業大學學報,2020,39(6):164-172

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        • 收稿日期:2020-03-02
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        • 在線發布日期: 2020-12-01
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