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      2. 黃顙魚朗格漢斯細胞的鑒定及其功能基因的表達分析
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        國家自然科學基金項目(31672683,31972819)


        Identification and functional gene expression analysis of Langerhans cells in yellow catfish (Pelteobagrus fulvidraco)
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          摘要:

          為研究黃顙魚朗格漢斯細胞的結構特點以及細菌感染對其功能相關基因表達的影響,采用免疫組化技術對黃顙魚免疫器官中朗格漢斯細胞進行鑒定,通過透射電鏡進一步觀察到黃顙魚朗格漢斯細胞的超微結構,并采用熒光定量PCR檢測鯰愛德華氏菌感染后黃顙魚免疫相關組織內朗格漢斯細胞功能相關基因IL-1β、TNF-α、IL-10和TLR-4的表達情況。結果顯示,黃顙魚頭腎和脾臟中具有朗格漢斯細胞的特異性分子標記CD207蛋白,且黃顙魚朗格漢斯細胞的結構與其他脊椎動物類似,即細胞質中大量的伯貝克顆粒圍繞著中心粒呈放射狀排列。在鯰愛德華氏菌感染后,朗格漢斯細胞功能相關基因IL-1β、TNF-α、IL-10和TLR-4在黃顙魚免疫器官內的表達量顯著上調。上述結果表明,黃顙魚含有朗格漢斯細胞,并且其可能參與了機體抗鯰愛德華氏菌感染的免疫反應。

          Abstract:

          To study the structural characteristics of Langerhans cells (LCs) and the effect of bacterial infection on the expression of functional genes of LCs in yellow catfish,the LCs in immune tissues of yellow catfish were identified by immunohistochemical (IHC) technique,the ultrastructure of LCs was further observed by transmission electron microscope (TEM) technique,and the expression levels of IL-1β,TNF-α,IL-10 and TLR-4 genes related to the function of LCs in the immunity related tissues of yellow catfish were detected by real-time quantitative PCR in this study. The results showed that the CD207 protein,a specific molecular marker for LCs,were detected in the head kidney and spleen,and the structure of the yellow catfish LCs was similar to that of other vertebrates,with granules arranged radially around the centriole in cytoplasm. The expression levels of the IL-1β,TNF-α,IL-10 and TLR-4 genes in the immunity related tissues of yellow catfish were significantly up-regulated after Edwardsiella ictaluri infection. The above results indicated that the LCs were distributed in yellow catfish,and they may participate in the body′s immune response against E. ictaluri infection.

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        王輝,李思思,吳志新,許荔立,田丁,陳孝煊.黃顙魚朗格漢斯細胞的鑒定及其功能基因的表達分析[J].華中農業大學學報,2020,39(6):173-179

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        • 收稿日期:2020-01-09
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        • 在線發布日期: 2020-12-01
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