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      2. 組氨酸及組胺對翹嘴鱖攝食調控的影響
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        現代農業產業技術體系專項 (CARS-46);華中農業大學大北農青年學者提升專項(2017DBN014); 武漢市青年科技晨光計劃(2017050304010318) 


        Effect of histidine and histamine on feeding regulation of Chinese perch (Siniperca chuatsi)
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          摘要:

          以翹嘴鱖(Siniperca chuatsi)為研究對象,將其分為3組,經腦室分別注射(ICV)磷酸鹽緩沖液(對照組)、組氨酸和組胺(試驗組),通過檢測翹嘴鱖24 h內的攝食量、食欲基因和氨基酸感知信號通路的變化來揭示組氨酸及組胺對魚類攝食調控的影響。結果顯示,腦室注射組氨酸和組胺后翹嘴鱖的攝食量在12 h相比于對照組顯著性下降,同時npy 、gcn2和atf4 mRNA的表達均顯著降低(P<0.05),而agrp和mtor mRNA的表達卻無顯著性變化(P>0.05)。離體水平,通過用不同濃度(1.0、2.0和3.0 mmol/L)的組氨酸和組胺處理鱖腦原代細胞后檢測促食欲基因的表達情況,結果顯示,與對照相比,1.0、2.0和3.0 mmol/L的組氨酸處理顯著抑制了鱖原代腦細胞npy mRNA 的表達(P<0.05),且隨著濃度的升高,這種抑制作用會增強;同時,1.0和3.0 mmol/L的組氨酸處理也顯著抑制了鱖原代腦細胞agrp mRNA 的表達(P<0.05),但不同濃度的組胺刺激并沒有引起鱖原代腦細胞npy和agrp mRNA 的表達變化。此外, 2.0 mmol/L的組氨酸刺激并不會引起鱖腦原代細胞mTOR信號通路下游關鍵蛋白p-S6的表達變化。以上結果提示:組氨酸和組胺會抑制翹嘴鱖的食物攝入,組氨酸可能是通過激活鱖腦組胺神經元來發揮作用,而這種抑制作用可能是通過下調促食欲因子的表達來抑制翹嘴鱖的食物攝入量。

          Abstract:

          In the present study,the effects of histidine and histamine on food intake were evaluated through intraventricular (ICV) administration in Chinese perch (Siniperca chuatsi),and the primary cells of Chinese perch were stimulated with different concentrations of histidine and histamine. The food intake changes were examined within 24 h after ICV,and the changes of the appetite genes and amino acid sensing signaling pathways were detected. The results showed that histidine and histamine significantly decreased food intake at 12 h post-injection,compared with the control group. Meanwhile,the mRNA expression of npy,gcn2 and atf4 were significantly decreased (P<0.05),whereas the mRNA expression of agrp and mtor were not significantly changed (P>0.05). At the cellular level,compared with the control group,1.0,2.0 and 3.0 mmol/L of histidine obviously inhibited the expression of the npy mRNA (P<0.05),and this inhibitory effect increased with increasing concentration. At the same time,1.0 and 3.0 mmol/L of histidine also significantly inhibited the expression of the agrp mRNA (P<0.05). In addition,different concentrations of histamine did not cause changes the npy and agrp mRNA expression (P>0.05). Besides,2.0 mmol/L of histidine didn’t significantly change the phosphorylation level of protein S6,the downstream signaling of mTOR pathway. Our results suggest that histidine and histamine can inhibit the food intake of Chinese perch. Histidine suppresses food intake through its conversion into neuronal histamine,and the inhibitory effect of histidine and histamine on food intake might be mediated through down-regulating the expression of appetite-promoting genes.

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        朱強勝,何珊,梁旭方,陳珂,鄒家明.組氨酸及組胺對翹嘴鱖攝食調控的影響[J].華中農業大學學報,2020,39(6):180-186

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        • 收稿日期:2020-01-14
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        • 在線發布日期: 2020-12-01
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