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      2. 組氨酸及組胺對翹嘴鱖攝食調控的影響
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        水產學院

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        華中農業大學大北農青年學者提升專項(2017DBN014); 武漢市青年科技晨光計劃(2017050304010318); 現代農業產業技術體系專項資金(CARS-46)


        Effect of Histidine and Histamine on Feeding Regulation of Chinese perch(Siniperca chuatsi)
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        College of fishery

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          摘要:

          為評估組氨酸和組胺對翹嘴鱖攝食調控的影響,對肉食性魚類翹嘴鱖(Siniperca chuatsi)腦室注射(ICV)組氨酸及組胺,并用不同濃度組氨酸和組胺刺激鱖原代腦細胞,統計腦室注射后翹嘴鱖24h內的攝食量的變化,檢測食欲基因和氨基酸感知信號通路的變化。結果顯示,腦室注射組氨酸和組胺后翹嘴鱖的攝食量在12 h相比于對照組顯著性下降,同時npy 、gcn2和atf4 mRNA的表達均顯著降低(P<0.05),而agrp和mtor mRNA的表達卻無顯著性變化(P>0.05)。離體水平,與對照相比,1.0、2.0和3.0 mmol/L的組氨酸顯著性抑制了npy mRNA 的表達(P<0.05),且隨著濃度的升高,這種抑制作用會增強;同時,1.0和3.0 mmol/L的組氨酸也顯著抑制了agrp mRNA 的表達(P<0.05),但不同濃度的組胺刺激并沒有引起npy和agrp mRNA 的表達變化(P>0.05)。此外, 2.0 mmol/L的組氨酸刺激并不會引起鱖原代腦細胞mTOR信號通路下游關鍵蛋白S6的表達變化(P>0.05)。以上結果提示:組氨酸和組胺會抑制翹嘴鱖的食物攝入,組氨酸可能是通過激活鱖腦組胺神經元來發揮作用,而這種抑制作用可能是通過下調促食欲因子的表達來抑制翹嘴鱖的食物攝入量。

          Abstract:

          In the present study, the effects of histidine and histamine on food intake were evaluated through intraventricular (ICV) administration in Chinese perch (Siniperca chuatsi), and the primary cells of Chinese perch were stimulated with different concentrations of histidine and histamine. This study examined the food intake changes within 24 hours after ICV, and detected the changes of appetite genes and amino acid sensing signaling pathways. The results showed that histidine and histamine significantly decreased food intake at 12 h post-injection, compared with the control group. Meanwhile, the mRNA expression of npy, gcn2 and atf4 were significantly decreased (P<0.05), whereas the mRNA expression of agrp and mtor were no significant change (P>0.05). At the cellular level, compared with the control group, 1.0, 2.0 and 3.0 mmol/L of histidine obviously inhibited the expression of npy mRNA (P<0.05), and this inhibitory effect increased with increasing concentration; At the same time, 1.0 and 3.0 mmol/L of histidine also significantly inhibited the expression of agrp mRNA (P<0.05). In addition, different concentrations of histamine did not cause changes in npy and agrp mRNA expression (P>0.05). Besides, 2.0 mmol/L of histidine was no significant change the phosphorylation level of protein S6, as the downstream signaling of mTOR pathway. Our results suggest that histidine and histamine can inhibit the food intake of Chinese perch. Histidine suppresses food intake through its conversion into neuronal histamine, and the inhibitory effect of histidine and histamine on food intake might be mediated through down-regulating the expression of appetite-promoting genes.

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        • 收稿日期:2019-12-14
        • 最后修改日期:2020-01-30
        • 錄用日期:2020-10-30
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