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      2. 三種芽孢桿菌菌劑對黃瓜枯萎病的防效及其作用機理初探
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        華中農業大學生命科學技術學院

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        國家自然科學基金項目(面上項目,重點項目,重大項目)


        Control effect of three Bacillus strains on cucumber fusarium wilt and its mechanism
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        College of Life Science and Technology,Huazhong Agricultural University

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          摘要:

          以含有黃瓜枯萎病的病原菌(尖孢鐮刀菌)的病土為材料,采用盆栽試驗研究了巨大芽孢桿菌B213、多粘類芽孢桿菌B207和枯草芽孢桿菌B204單獨或聯合施用對黃瓜枯萎病的防效,并對其作用機理進行了初步分析。結果顯示:3種芽孢桿菌單獨或組合施用后均可有效抑制黃瓜枯萎病的發生,并顯著增加黃瓜植株的生物量,且三菌劑聯用>雙菌劑聯用>單菌劑施用的效果。在黃瓜幼苗移栽后35 d,與對照組相比,三菌劑聯用組黃瓜枯萎病的發病率降低了79.99%;植株干質量提高了6.92倍;植株抗性酶如苯丙氨酸解氨酶、多酚氧化酶、過氧化物酶活性分別提高了60.02%、28.90%和108.60%;根際土壤尖孢鐮刀菌的數量下降了2個數量級。該防病促生效果可能與這3種芽孢桿菌對病原菌的拮抗作用、溶磷能力以及吲哚乙酸、蛋白酶、幾丁質酶和嗜鐵素的分泌能力有關。

          Abstract:

          The diseased soil containing pathogens of Fusarium oxysporum f. sp. was used as the material. Using the pot experiment to explore the control effects of Bacillus megaterium B213, Bacillus polymyxa B207, and Bacillus subtilis B204 alone or in combination on cucumber fusarium wilt and its mechanism. The results showed that the three microbial agents alone or in combination could effectively inhibit the occurrence of cucumber fusarium wilt and significantly increase the biomass of cucumber. The effect of the two microbial agents in combination was better than that of single microbial agent. And the effect of the three microbial agents in combination was better than that of the two microbial agents. 35 days after transplanting cucumber seedlings, compared with the control group, the incidence of cucumber fusarium wilt in the three-agent combination group was reduced by 79.99%; the dry weight of cucumber plants was increased by 6.92 times; the activities of plant resistance enzymes, such as acid lyase, polyphenol oxidase, and peroxidase increased by 60.02%, 28.90%, and 108.60% respectively; the number of rhizosphere soil Fusarium oxysporum decreased by 2 orders of magnitude. These effects may be related to the antagonism of these three Bacillus agents to pathogenic bacteria, phosphorus dissolving ability, and secretory ability of indoleacetic acid, protease, chitinase and ferrophilin.

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        • 收稿日期:2019-12-16
        • 最后修改日期:2020-01-10
        • 錄用日期:2020-06-08
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