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      2. 植物病原絲狀真菌寄生性與RGS蛋白的關系研究
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        西南林業大學

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        中圖分類號:

        S435.1

        基金項目:

        國家自然科學基金項目(31960314);云南省應用基礎研究計劃項目(2018FG001-028)


        The relationship between parasitism and RGS protein in plant pathogenic filamentous fungi
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        Southwest Forestry University

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          摘要:

          RGS作為G蛋白信號轉導途徑的負調控因子,在植物病原菌的致病性和有性生殖調控方面發揮著重要作用。研究植物病原真菌寄生性與RGS的關系,為今后深入開展不同寄生性真菌中不同類型RGS的功能解析奠定一定的理論基礎。 以49個全基因組序列已經釋放的絲狀真菌為研究對象,利用OrthoVenn2進行RGS同源基因比對,根據釀酒酵母的4個RGS氨基酸序列在線BLASTp比對以及NCBI數據庫關鍵詞檢索等方法,并通過SMART進行保守結構域分析。結果表明,49個真菌中共有229個RGS,每種真菌中所含有RGS的數量范圍為3-9個;根據RGS中保守結構域進行分類,可以劃分為DEP-RGS、RGS-TM、PXA-RGS-PX、RGS、RGS-PAS-PAC、TM-RGS等6種,其中,具有RGS-PAS-PAC和TM-RGS這2種特殊保守結構域的RGS主要集中在半活體營養型病原菌和死體營養型病原菌;進一步對229個RGS進行遺傳關系分析,發現具有同種保守結構域的RGS親緣關系較近。本研究發現植物病原絲狀真菌具有6種類型的RGS蛋白,其中死體營養型真菌和半活體營養型真菌RGS蛋白的種類及數量均高于活體營養型真菌。

          Abstract:

          Regulators of G-protein Signaling as a negative regulator of G protein signal transduction pathway, plays an important role in the pathogenicity and sexual reproduction of plant pathogens. Study the relationship between parasitism and RGS protein in plant pathogenic fungi will lay a theoretical foundation for further functional analysis of different types of RGS in different parasitic fungi. forty-nine filamentous fungi with the whole genome sequence were used as research objects. OrthoVenn2 was used to perform RGS homologous gene alignment, online BLASTp alignment based on the 4 RGS amino acid sequences of Saccharomyces cerevisiae, and using keyword search in NCBI database, etc., and through SMART conservative domain analysis, the results showed that there were 229 RGS in 49 fungi, and the number of RGS in each fungus ranged from 3 to 9. According to the conserve domains in RGS, above RGS protein can be divided into DEP-RGS, RGS-TM, PXA-RGS-PX, RGS, RGS-PAS-PAC, TM-RGS and other six types, of which RGS with two special conserved domains, RGS-PAS-PAC and TM-RGS, are mainly concentrated in semi-living vegetative pathogens and dead vegetative pathogens, further genetic analysis of 229 RGS proteins showed that RGS with the same conserved domain was closely related. This study found that plant pathogenic filamentous fungi have six types of RGS proteins, among which the types and quantities of RGS proteins of hemibiotrophic and necrotrophic fungi were higher than that in biotrophic fungi.

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        • 收稿日期:2020-01-09
        • 最后修改日期:2020-06-03
        • 錄用日期:2020-10-21
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