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      2. 黃顙魚朗格漢斯細胞的鑒定及功能基因的表達分析
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        華中農業大學

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        國家自然科學基金項目(面上項目,重點項目,重大項目)


        The identification of Langerhans cell and the expression levels of gene-relatived to funcation of Langerhans cell in yellow catfish (Pelteobagrus fulvidraco)
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        huazhong agricultural university

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          摘要:

          朗格漢斯細胞作為樹突狀細胞的亞型之一,主要分布于哺乳動物皮膚表皮和部分粘膜組織中,在抗細菌感染過程中發揮重要作用。為研究黃顙魚朗格漢斯細胞的結構特點以及細菌感染對其功能相關基因表達的影響,采用免疫組化技術在黃顙魚頭腎和脾臟中檢測到CD207陽性細胞,并通過透射電鏡進一步觀察到黃顙魚朗格漢斯細胞的超微結構。熒光定量PCR結果表明,鯰愛德華氏菌能夠誘導黃顙魚免疫器官內朗格漢斯細胞功能相關基因的顯著上調。上述結果表明,黃顙魚朗格漢斯細胞的結構與其他脊椎動物類似,并且參與了機體抗鯰愛德華氏菌誘導的免疫反應。本試驗不僅有助于我們分析朗格漢斯細胞在脊椎動物中的進化特點,更為黃顙魚適應性免疫系統的研究提供一定的理論基礎。

          Abstract:

          Langerhans cells, one of the subtypes of Dendritic cells, are mainly distributed in mammalian skin epidermis and some mucosal tissues, and play an important role in against bacterial infection. In order to study the structural characteristics of Langerhans cells and the effect of bacterial infection on the expression of gene-related the function of Langerhans cells, CD207 positive cells were detected in the head kidney and spleen of yellow catfish by immunohistochemistry, and the ultrastructure of Langerhans cells was further observed by transmissionSelectronSmicroscope. The results of real-time quantitative PCR showed that Edwardsiella ictaluri could induce significant up-regulation of gene-related the function of Langerhans cells in the immune organs of yellow catfish. The above results indicated that the structure of yellow catfish Langerhans cells was similar to that in other vertebrates and participate in the immune response induced by E.ictaluri. This study will not only help us analyze the evolutionary characteristics of Langerhans cells in vertebrates, but provides a theoretical basis for the research of the adaptive immune system of yellow catfish.

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        • 收稿日期:2020-01-09
        • 最后修改日期:2020-06-05
        • 錄用日期:2020-10-30
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