Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences
從湖北潛江患病克氏原螯蝦肝胰腺內分離到純化到一株致病菌,經過生理生化、16s rRNA序列測定和進化樹構建對該菌株進行了鑒定,通過瓊脂擴散法測定了分離株的藥物敏感性,通過肉湯微量稀釋法測定了抗菌藥物的最小抑菌濃度。結果發現,該菌株具有與肺炎克雷伯菌一致的生理生化特征,16s rRNA測序和進化樹分析發現該分離株與肺炎克雷伯菌的親緣關系較近,其基因相似度達到99%以上,因此將該菌株鑒定為肺炎克雷伯菌。通過藥敏試驗發現該菌株僅對頭孢噻肟和多粘菌素B敏感,該分離株為多重耐藥菌株。
A pathogenic bacterium was isolated from diseased Procambarus clarkii in Qianjiang, Hubei province. The strain was identified by physiological and biochemical assay, 16s rRNA sequence determination and phylogenetic analysis. Then the susceptibility to antibiotics was performed by agar diffusion method, the minimum inhibitory concentrations (MICs) were evaluated by micro-dilution method. The results showed that the physiological and biochemical characteristics similar to Klebsiella pheumonia. The 16s rRNA sequencing and phylogenetic analysis showed that the strain had close genetic relationship shared over 99% homologies with Klebsiella pheumonia. Therefore, the strain was identified as Klebsiella pheumonia. According to the results of susceptibility results, the strain was sensitive to cefotaxime and aerosporin among all the tested antibiotics These results indicated that the strain was a multi-resistant Klebsiella pheumonia strain.