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      2. 雌激素受體基因對泥鰍雌雄和倍性生長差異的表達調控作用
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        華中農業大學

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        中圖分類號:

        Q 959.54

        基金項目:

        國家重點研發計劃藍色糧倉創新專項(2018YFD0900205)


        Regulation of ER genes expression on growth differences of sexes and diploids in Misgurnus anguillicaudatus
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        Huazhong Agricultural University

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          摘要:

          為探討雌激素受體基因與魚類雌雄及倍性生長差異之間的相關性,選取雌性生長快于雄性、且四倍體生長快于二倍體的泥鰍為研究對象,首先克隆泥鰍雌激素受體基因,獲得ERα (1 821 bp)、ERβ1(1 008 bp)和ERβ2(1 197 bp)的cDNA序列,氨基酸序列比對發現泥鰍ER分別與鯉科魚類相應亞型具有較高的同源性。采用qRT-PCR檢測ER基因在二倍體泥鰍性成熟前后5個關鍵時期的表達情況及二、四倍體相關組織間的差異表達。結果顯示,3個基因在雌雄個體性成熟前后腦、肌肉、肝臟和性腺組織中均存在差異表達,其中ERα 在泥鰍雄性個體腦、肌肉和性腺組織中的表達量顯著高于雌性(P<0.05),而在雌性肝臟組織中的表達量普遍高于雄性(P<0.05);ERβ1和ERβ2在精巢組織中的表達量一直處于較高水平。泥鰍二、四倍性間的比較結果顯示,3個ER基因在泥鰍二倍體腦組織中的表達量顯著高于四倍體的表達量,且雄性的表達量均高于雌性,其中ERα表達差異最為顯著。本研究結果表明,無論是針對倍性生長差異還是雌雄生長差異,ERα均表現為在生長較慢(二倍體、雄性)個體腦組織中有相對高表達,推測腦組織中ERα 基因對魚類個體生長速度具有一定的抑制調控作用。

          Abstract:

          In order to investigate the relationship between estrogen receptor genes and growth differences in sexes and ploidy of fish, loach(Misgurnus anguillicaudatus)with females growing faster than males and tetraploids growing faster than diploids were selected as the research object in this study. We firstly cloned the cDNA sequences of three estrogen receptor genes of loach, including ER (1821 bp), ERβ1 (1008 bp) and ERβ2 (1197 bp). Amino acid sequence alignment revealed that loach ER genes have higher homology with corresponding subtypes of carps. Quantitative RT-PCR was used to detect the expression of ER genes in five growth stages of diploid loach as well as diploid and tetraploid adults. The results showed that three genes were differentially expressed in brain, muscle, liver, and gonadal tissues of male and female individuals before and after sexual maturity. Among them, the expression of ERα in brain, muscle and gonad tissues of male individuals was significantly higher than that of females (P<0.05), and the expression in female liver tissues was generally higher than that in males (P<0.05). The expression levels of ERβ1 and ERβ2 in testis tissue were always in high level. Comparison results between different ploidy showed that the expression of three ER genes in the diploid brain tissue were significantly higher than that of the tetraploids, and the expression levels of males were higher than females, with the expression of ERα being the most significant. The results of this study indicated that regardless of the difference between ploidy and sex growth, ERα appears to have relatively high expression in the brain tissue of slower growing (diploid, male) individuals. Therefore, we speculate that the ERα gene in brain tissues might have a certain inhibitory effect on the growth rate of individual fish.

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        • 收稿日期:2020-03-02
        • 最后修改日期:2020-09-26
        • 錄用日期:2020-10-30
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