National Science and Technology Major Project；National Key Research and Development Program of China during the 13th Five-Year Plan Period；The Fundamental Research Funds for the Central Universities
為探究PGF2α信號對水牛卵母細胞和胚胎發育的作用，利用免疫熒光和熒光定量檢測了PGF2α受體（PTGFR）在水牛卵母細胞和胚胎早期發育過程中的表達規律；然后在卵母細胞體外成熟液、胚胎培養發育液中分別添加不同濃度的AL-8810，檢測其對水牛卵母細胞成熟效率以及胚胎發育的效率的影響。結果顯示，PTGFR蛋白在水牛卵母細胞及胚胎中廣泛分布，在4-16-細胞期胚胎階段，PTGFR基因表達水平顯著高于未受精的卵母細胞和桑椹胚（P＜0.01）；AL-8810對水牛卵母細胞核成熟效率無顯著影響（P＞0.05），但高濃度處理組（1 600 nmol/L）顯著降低卵母細胞早期凋亡率（P＜0.05），100和800 nmol/L的AL-8810可顯著提高體外胚胎16-細胞期的發育效率（P＜0.05），800 nmol/L組桑葚胚形成率最高。以上研究結果表明：抑制前列腺素PGF2α信號可有效提高水牛卵母細胞體外成熟質量與早期胚胎發育潛力。
The purpose of this study was to explore the effect of PGF2α signal on the oocyte and early embryo in buffalo, the expression patterns of PTGFR in buffalo oocytes and early embryos development were determined by immunofluorescence and fluorescence quantification. Subsequently, the effects of AL-8810 on the maturation efficiency of oocytes and the efficiency of embryonic development in buffalo were studied by adding different concentrations of AL-8810 into the oocyte maturation medium and embryonic development medium. The results showed that PTGFR protein was widely distributed in buffalo oocytes and embryos, and the expression level of PTGFR gene was significantly higher in 4-16 cell stage than in unfertilized oocytes and morulae (P＜0.01). AL-8810 had no significant differences effect on the nuclear maturation efficiency of buffalo oocytes (P＞0.05), however, high concentration treatment group(1 600 nmol/L) significantly reduced the early apoptosis rate of buffalo oocytes (P＜0.05). AL-8810 at the concentration of 100 and 800 nmol/L significantly improved embryonic development efficiency in 16 cell stage, and the morula formation rate was the highest in the 800 nmol/L group. The above results indicated that inhibition of prostaglandin PGF2α signal effectively improved in vitro maturation quality of oocytes and early embryos development potential in buffalo.