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      2. 千里光石油醚提取物對LPS激活的炎癥反應的抑制效應研究
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        中南民族大學

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        國家自然科學基金資助項目(31371307)


        Anti-inflammatory effect of petroleum ether extract from Senecio scandens on LPS-stimulated RAW264.7 cells
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          摘要:

          為探討千里光抗炎作用及機制,以千里光石油醚提取物(PEESS)為材料,以脂多糖(LPS)誘導的RAW264.7小鼠單核巨噬細胞為炎癥模型,通過CCK-8法檢測細胞增殖、Griess法檢測NO含量、RT-PCR法及Western blot法檢測炎癥相關因子表達,研究了PEESS的抗炎作用及可能的分子機制。結果顯示,0~80 μg/mL的PEESS對巨噬細胞增殖無明顯影響,但能顯著抑制LPS誘導的RAW264.7細胞NO的分泌,并呈濃度依賴性;對炎癥相關因子基因mRNA表達檢測結果表明PEESS能顯著抑制LPS 致RAW264.7 細胞炎癥模型中IL-1β及IL-6的表達;同時,PEESS還顯著降低了p-NF-κB p65及 p-p44/p22 MAPK蛋白的表達。綜上,千里光石油醚提取物對LPS 誘導的RAW264.7 細胞炎癥模型具有較好抗炎效應,其作用發揮可能與其抑制NO、IL-1β和IL-6等炎癥介質的分泌及NF-κB 信號通路和MAPK信號通路的活化有關,為將千里光應用于動物抗炎及探究其具體作用機制奠定了基礎。

          Abstract:

          in order to explore the anti-inflammatory effect and underlying mechanism of Senecio scandens, Petroleum ether extract of Senecio scandens(PEESS)was used to treat the lipopolysaccharide(LPS)-induced inflammation response in mouse mononuclear macrophages RAW264.7. The cell proliferation were detected by CCK-8, and the secretion of NO were detected by Griess method, and the expression of inflammation-associated factors were detected by RT-PCR and Western blot. The results showed that PEESS at 0~80 μg/mL had no significant effect on the proliferation of RAW264.7. However, it can significantly inhibit the secretion of NO in LPS induced RAW264.7 cells by a concentration-dependent manner. And the expression of IL-1β and IL-6 were significantly inhibited by PEESS in LPS induced RAW264.7 cells. Meanwhile, PEESS also significantly reduced the level of p-NF-κB p65 and p-p44/p22 MAPK proteins. In conclusion, PEESS may participate in the process of anti-inflammatory response by inhibiting the expression of some pro-inflammatory mediators such as NO, IL-1β and IL-6, and the activation of the NF-κB and MAPK signaling pathways. This study lays a foundation for the application of Senecio scandens in cuing some inflammatory disease of animals.

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        • 收稿日期:2020-04-20
        • 最后修改日期:2020-07-18
        • 錄用日期:2020-10-30
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