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      2. 大豆與叢枝菌根真菌共生建立及菌根檢測方法的探究
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        華中農業大學

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        國家重點基礎研究發展計劃(973計劃)


        Establishment of symbiosis between soybean and arbuscular mycorrhizal (AM) fungi and study of mycorrhizal detection method
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          摘要:

          利用盆栽培養的方法,研究四種培養基質對不同品種大豆與AM真菌(Rhizophagus irregularis)共生的影響,比較parker純黑墨水和臺盼藍兩種染色劑對大豆菌根的染色效果,探究放大網格交叉法和Trouvelot五級分級法在大批量菌根共生指標檢測中的應用。結果表明:蛭石和土(V:V=4:1)這種基質更有利于AM真菌對大豆的侵染,該基質中菌根的叢枝豐度、根內菌絲密度、泡囊豐度均顯著高于其他基質,可以作為大豆與AM真菌共生盆栽培養的最佳培養基質。對于大批量的菌根共生指標檢測來說,墨水染色技術和放大網格交叉法是目前最優的菌根染色和真菌指標檢測方法。

          Abstract:

          The effects of four Culture medium on the symbiosis of different varieties of soybean and AM fungi (Rhizophagus irregularis) were studied by pot culture. The difference between black ink and trypan blue staining methods and the application of the magnified grid line and the five-class methods in mycorrhizal symbiosis structures detection were also compared in this research.The results show that vermiculite and soil (V: V = 4: 1) are more conducive to the infection of AM fungi with soybean. The arbuscular abundance, hypha density and vesicle abundance in this culture substrate are significant higher than others, so it can be used as the best culture substrates for soybean and AM fungi symbiosis. For large-scale mycorrhizal symbiosis structures detection, ink staining and the magnified grid line method are the best methods in currently.

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        • 收稿日期:2020-05-10
        • 最后修改日期:2020-06-10
        • 錄用日期:2020-09-14
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