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      2. 獼猴桃采后軟腐病病原鑒定及香芹酚對其控制效果研究
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        華中農業大學食品科學學院

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        S482.99

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        國家重點研發計劃 ,項目編號 2019YFD1002300


        Identification of Soft-rot Pathogens in Postharvest Kiwifruits and its Control by Carvacrol
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          摘要:

          獼猴桃果實軟腐病是我國獼猴桃采后貯運期間和銷售過程中危害最為嚴重的病害,給生產帶來巨大損失。本文依據傳統真菌形態學觀察法和rDNA-ITS序列分析技術對發病獼猴桃果實中的致病菌進行病原的分離鑒定,并研究香芹酚對分離得到的獼猴桃軟腐病菌的控制效果。結果表明,引起獼猴桃采后果實軟腐病的主要致病菌為葡萄座腔菌(Botryosphaeria dothidea)與擬莖點霉菌(Phomopsis vaccinii),其次為灰葡萄孢菌(Botrytis cinerea)、層出鐮刀菌(Fusarium proliferatum)和鏈格孢菌(Alternaria alternata)。香芹酚對獼猴桃軟腐病菌表現出良好的抑菌活性,其中對主要致病菌葡萄座腔菌和擬莖點霉菌的抑制作用尤為顯著,半抑制濃度(EC50)分別為12.57、9.09μL/L。同時,香芹酚有效控制模擬銷售環境下獼猴桃果實軟腐病的發生,放置第8d時處理組發病率僅為對照果實的50.17%,且對獼猴桃果實的品質指標影響不顯著(P>0.05),因此,香芹酚在控制獼猴桃果實采后軟腐病方面具有潛在的應用價值。

          Abstract:

          The kiwifruits soft rot is the most serious disease during the storage and transportation of kiwifruits, which causing great economic losses. In this study, the pathogenic fungus causing kiwifruit soft rots were isolated and identified by examining the morphological and microscopic characteristics, combined with the results of pathogenicity testing and ITS (internal transcribed spacer) sequencing, and the inhibitory effect of carvacrol on kiwifruits soft rot was further evaulated. Two species, Botryosphaeria dothidea and Phomopsis vaccinii, were identified as the main pathogens causing kiwifruit postharvest soft rots, the other three species followed by Botrytis cinerea, Fusarium proliferatum and Alternaria alternata. Carvacrol had significant antifungal activity against eight soft rot pathogens,especially for B. dothidea and Phomopsis sp.,which half effective concentration (EC50) were 12.57 and 9.09 μL / L, respectively. Simultaneously, carvacrol effectively reduced the incidence of soft rot in kiwifruit under simulatedsales environment, which was only 50.17% compared with the control group, and had no significant effect on fruit quality(P > 0.05). Therefore, carvacrol has great potential to control soft rot of postharvest kiwifruit.

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        • 收稿日期:2020-05-11
        • 最后修改日期:2020-06-10
        • 錄用日期:2020-09-14
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