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      2. 莖瘤芥BXL基因家族的全基因組鑒定和在瘤狀莖膨大過程中的表達分析
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        長江師范學院

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        國家自然科學基金項目(31701928)、重慶市科技局基礎科學與前沿技術研究專項(cstc2017jcyjAX0226)、重慶市教委科學技術研究項目(KJ1712305)、長江師范學院校級科研項目(2016KYQD19和2016XJQN07)


        Genome?wide Identification of BXL Family Genes in Brassica juncea var. tumida and Their Expression Analysis During Stem Swelling
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          摘要:

          為深入解析BXL家族基因在莖瘤芥發育中的功能,利用生物信息學方法從莖瘤芥全基因組鑒定出16個BjuBXL并系統分析了BjuBXL家族蛋白的理化性質、基因結構、保守基序、染色體定位和在莖膨大過程中的表達。結果表明:16個BjuBXL基因分為7類,共2個亞族,其中BjuBXL1-1~1-4與擬南芥中參與細胞壁代謝和植物發育的AtBXL1親緣關系相近;7個BjuBXL定位于A亞基因組的5條染色體上,8個定位于B亞基因組的4條染色體上;熒光定量PCR分析表明BjuBXL1-1和BjuBXL1-2在莖膨大過程中均明顯上調,而且在莖的內髓區(P2-P4)表達量均顯著高于外髓區(P1),可能參與莖膨大過程中莖內髓區的細胞壁代謝。

          Abstract:

          Beta-D-xylosidase (BXL) is the key rate-limiting enzyme for the complete degradation of xylan, which is the main component of hemicellulose in the plant cell wall. In this study, 16 genes encoding putative BXL proteins were identified in Brassica juncea genome. Protein physicochemical properties, gene structure and conserved motifs, chromosomal distribution and gene expression during stem swelling of BjuBXL family genes were analyzed. The results showed that 16 BjuBXL genes were classified into seven categories and formed two subfamilies according to the phylogenetic tree analysis. Subfamily 1 included BjuBXL1~5 and Subfamily 2 included BjuBXL6 and BjuBXL7. BjuBXL1-1~1-4 were closely related to AtBXL1, which was involved in the Arabidopsis cell wall metabolism and plant development. Multiple alignments of the 16 BjuBXL protein sequences revealed that all of them contained the conserved WGR and KH motifs that are associated with substrate binding and conserved amino acids at two key active sites for xylose hydrolysis. Sixteen BjuBXL genes coded 769 to 984 amino acids, which corresponding 83.37 to 109.32 kD molecular weight protein. The grand average of hydropathicity (GRAVY) score of the 16 BjuBXL proteins was predicted from -0.273 to -0.049, indicating these proteins are hydrophilic. According to gene structure and conserved motifs analysis, subfamily 1 of BjuBXL contained 5~11 exons and subfamily 2 contained 3~5 exons, and all the BjuBXL proteins contain ten conserved motifs. Chromosomal distribution analyses showed that 7 BjuBXL genes were located in 5 chromosomes of A subgenomes and 8 BjuBXL genes are located in 4 chromosomes of B subgenomes. The expression patterns of 16 BjuBXL genes during stem swelling was obtained from the RNA-seq data, 9 BjuBXL genes showed noticeable expression changes during the stem swelling process, while other 7 BjuBXL genes were nearly not expressed. The expression of BjuBXL1-1 and BjuBXL 1-2 were found notably correlated with stem swelling. Furthermore, real-time PCR results revealed that the transcriptional levels of BjuBXL1-1and BjuBXL1-2 were up-regulated during stem swelling and were significantly higher in the inner pith (P2~P4) than in regions of the outer pith of swollen stem (P1). Our results suggest that BjuBXL1-1 and BjuBXL1-2 may play an essential role in the inner pith cell wall metabolism during stem swelling. This study laid a foundation for further investigations of the function of BXL family genes in B. juncea var. tumida.

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        • 收稿日期:2020-05-28
        • 最后修改日期:2020-09-21
        • 錄用日期:2020-09-22
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