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      2. 1株克氏原螯蝦摩氏摩根菌的分離鑒定及聯合藥敏試驗
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        中國水產科學研究院長江水產研究所

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        國家自然科學基金,湖北省技術創新專項重大項目


        Identification and synergistic susceptibility test of pathogenic Morganella morganii isolated from diseased Procambarus clarkii
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          摘要:

          從患病克氏原螯蝦肝胰腺內分離到1株病原菌,通過對其生理生化特征分析、16s rRNA序列測定和進化樹分析對分離株進行了鑒定;測定了分離株對20種抗菌藥物的敏感性及抗菌藥物與天然化合物聯用后的協同抑菌作用。結果發現,該菌株的生理生化特征與摩氏摩根菌基本一致,進一步通過通過測序和進化樹分析發現其與摩氏摩根菌的親緣關系較近,因此該菌株鑒定為摩氏摩根菌。藥敏試驗發現該菌株對恩諾沙星、氟苯尼考和新霉素等7種藥物高度敏感,對卡那霉素中等敏感,對多西環素、鏈霉素和四環素等12種藥物耐藥。此外,聯合抑菌試驗發現多西環素與二氫辣椒堿聯用后具有協同抑菌作用。以上研究表明,本次從克氏原螯蝦肝胰腺中分離的病原菌為耐藥性摩氏摩根菌,可選用氟苯尼考、恩多沙星、新霉素或二氫辣椒堿和多西環素的復方制劑進行治療。

          Abstract:

          A pathogenic bacterium was obtained from hepatopancreas of diseased Procambarus clarkii (P. clarkii). The isolated was determined by physiological and biochemical analysis, 16s rRNA sequencing and phylogenetic analysis. Then the sensitivity of the strain and synergistic susceptibility were determined by agar diffusion method and checkerboard assay. The results showed that physiological and biochemical characters similar to Morganella morganii (M. morganii) strain, then 16s rRNA sequence determination and phylogenetic analysis showed that the isolate showed close genetic relationship to M. morganii. Taken together, the strain was identified as a M. morganii strain. The susceptibility study showed that the strain was sensitive to enrofloxacin, florfenicol, neomycin and other 4 kinds of antibiotics, intermediate to kanamycin, resistant to doxycycline, streptomycin, tetracycline and other 9 kinds of antibiotics. Moreover, the synergistic susceptibility showed that doxycycline combined with dihydrocapsaicin had synergistic effect. Taken together, the results demonstrated that the strain isolated from hepatopancreas of diseased P. clarkii is a resistant M. morganii. Florfenicol, enrofloxacin, neomycin and doxycycline combined with dihydrocapsaicin can be chosen as candidate in treating M. morganii infections.

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        • 收稿日期:2020-08-06
        • 最后修改日期:2020-12-11
        • 錄用日期:2020-12-11
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