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      2. 廣陳皮中多甲氧基黃酮提取物對脂多糖誘導小鼠急性肺損傷的保護作用
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        華中農業大學食品科學技術學院

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        國家自然科學基金項目(面上項目,重點項目,重大項目)


        Protective Effect of Polymethoxyflavones in Chenpi on Lipopolysaccharide-induced Acute Lung Injury in Mice
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          摘要:

          采用D101大孔吸附樹脂從廣陳皮中獲得多甲氧基黃酮提取物,高速逆流色譜得到川陳皮素和桔皮素,高效液相色譜進行純度鑒定,化合物結構經質譜和核磁共振氫譜鑒定。通過鼻腔滴注脂多糖法建立小鼠急性肺損傷模型,以250mg/kg多甲氧基黃酮提取物組和25、50、100mg/kg低中高川陳皮素組連續灌胃7d,通過小鼠肺組織病理學變化、肺損傷評分、肺組織濕/干重比、髓過氧化物酶活性(MPO)、丙二醛含量(MDA)、谷胱甘肽過氧化物酶(GSH-PX)活性和細胞因子表達量等指標評價多甲氧基黃酮提取物和川陳皮素的抗氧化和抗炎作用。研究結果發現,多甲氧基黃酮提取物組和川陳皮素中劑量組能顯著緩解肺組織損傷,降低肺組織濕/干重比、MPO活性、MDA含量和促炎細胞因子的含量,提高GSH-PX活性,且川陳皮素中劑量組與10mg/kg地塞米松組對于急性肺損傷小鼠的抗氧化和抗炎效果相近。以上研究結果表明,多甲氧基黃酮提取物和川陳皮素具有抗氧化和抗炎作用,對急性肺損傷具有一定的保護作用。

          Abstract:

          D101 resin was used to obtain polymethoxyflavones extracts from the Chenpi . High-speed countercurrent chromatography was used to obtain nobiletin and tangeretin. The purity was identified by high performance liquid chromatography. The structure was identified by mass spectrometry and 1H NMR. The mice model of acute lung injury was constructed by intranasal instillation of LPS in the exprienment.Continuous gastric gavage with 25, 50, 100mg/kg low , medium,high-nobiletin group and 250mg/kg PMFs group for 7d.The anti-oxidant and anti-inflammation of PMFs and NOB was evaluated by assessing pulmonary histopathological changes,lung injury score,the wet weight to dry weight ratio, MPOactivity, MDA content, GSH-PX activity,the cytokine content of lung tissue.The results showed that the PMFs group and middle-NOB group can significantly relieve lung tissue damage, reduced the wet/dry weight ratio, MPO activity, MDA content and pro-inflammatory cytokine content, increased GSH-PX activity.The anti-oxidation and anti-inflammatory effects of the middle-NOB group and the 10 mg/kg dexamethasone group on acute lung injury mice are similar. The above results indicated that PMFs extracts and NOB have anti-oxidant and anti-inflammatory effects, and have certain protective effects on acute lung injury.

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        • 收稿日期:2020-09-29
        • 最后修改日期:2020-11-23
        • 錄用日期:2020-11-25
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