D101 resin was used to obtain polymethoxyflavones extracts from the Chenpi . High-speed countercurrent chromatography was used to obtain nobiletin and tangeretin. The purity was identified by high performance liquid chromatography. The structure was identified by mass spectrometry and 1H NMR. The mice model of acute lung injury was constructed by intranasal instillation of LPS in the exprienment.Continuous gastric gavage with 25, 50, 100mg/kg low , medium,high-nobiletin group and 250mg/kg PMFs group for 7d.The anti-oxidant and anti-inflammation of PMFs and NOB was evaluated by assessing pulmonary histopathological changes,lung injury score,the wet weight to dry weight ratio, MPOactivity, MDA content, GSH-PX activity,the cytokine content of lung tissue.The results showed that the PMFs group and middle-NOB group can significantly relieve lung tissue damage, reduced the wet/dry weight ratio, MPO activity, MDA content and pro-inflammatory cytokine content, increased GSH-PX activity.The anti-oxidation and anti-inflammatory effects of the middle-NOB group and the 10 mg/kg dexamethasone group on acute lung injury mice are similar. The above results indicated that PMFs extracts and NOB have anti-oxidant and anti-inflammatory effects, and have certain protective effects on acute lung injury.