D101 resin was used to obtain polymethoxyflavones extracts from the Chenpi . High-speed countercurrent chromatography was used to obtain nobiletin and tangeretin. The purity was identified by high performance liquid chromatography. The structure was identified by mass spectrometry and ¹H NMR. The mice model of acute lung injury was constructed by intranasal instillation of LPS in the exprienment.Continuous gastric gavage with 25, 50, 100mg/kg low , medium,high-nobiletin group and 250mg/kg PMFs group for 7d.The anti-oxidant and anti-inflammation of PMFs and NOB was evaluated by assessing pulmonary histopathological changes,lung injury score,the wet weight to dry weight ratio, MPOactivity, MDA content, GSH-PX activity,the cytokine content of lung tissue.The results showed that the PMFs group and middle-NOB group can significantly relieve lung tissue damage, reduced the wet/dry weight ratio, MPO activity, MDA content and pro-inflammatory cytokine content, increased GSH-PX activity.The anti-oxidation and anti-inflammatory effects of the middle-NOB group and the 10 mg/kg dexamethasone group on acute lung injury mice are similar. The above results indicated that PMFs extracts and NOB have anti-oxidant and anti-inflammatory effects, and have certain protective effects on acute lung injury.