1000-2421 2011 30 2 249 259 1000-2421(2011)02-0249-11 article 植物葉綠體基因工程研究進展 Progress in chloroplast transformation in plants 植物葉綠體基因工程與細胞核基因工程相比,具有許多獨特的優勢,如能夠實現外源基因特異整合及高效表達、多基因共表達、外源基因不會隨花粉擴散、沒有位置效應和基因沉默等。目前已在16種植物中成功獲得葉綠體轉基因植株,改良了植物的農藝性狀,特別是在煙草葉綠體中高效表達了40多種外源蛋白,包括多種抗體和疫苗。盡管如此,這項技術目前尚未用于主要糧食作物的性狀改良。本文綜述了植物葉綠體基因工程的原理、技術、應用、難點及進展。 Chloroplast transformation in plants has many advantages over nuclear transformation.Proteins in chloroplasts can be expressed at high levels with proper folding and disulfide bonds as the cells of higher plants contain a large number of chloroplast genomes.Multiple genes can be co-expressed in chloroplast genomes.Furthermore,chloroplast genes are inherited in a strictly maternal fashion in most angiosperm plant species,and this minimizes the possibility of out-crossing transgenes to related weeds or species.In addition,gene silencing,position effects and random integration have not been reported in chloroplast transformation.Although chloroplast transformation is very attractive,this technology is not as widely used as nuclear transformation.It has been mostly focused on 16 plants species,especially tobacco in which many proteins has been expressed including vaccines and antibodies.In this review we briefly summarize the rationales,methodologies,applications,bottlenecks and prospects of this promising genetic engineering technology for chloroplasts. 質體轉化; 葉綠體; 篩選標記基因; 植物生物反應器 plastid transformation; chloroplast; selective marker gene; plant bioreactor 程 琳,瞿 波,李和平,廖玉才 廖玉才 /hznydxzr/article/abstract/20110224
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