1000-2421 2016 35 1 81 85 article 解鉀菌解鉀效率檢測方法的比較 Comparing different methods of detecting potassium solubilizing efficiency with potassium solubilizing bacteria 以中慢生根瘤菌S-15和類芽胞桿菌S-17作為供試菌株,采用細胞破碎、NH4OAc浸提、H2O2溶液消煮及不作任何前處理等4種方法,利用火焰光度計檢測解鉀菌發酵液中K+含量,并計算解鉀菌在培養基中的解鉀效率。結果表明,配制的3種鉀系列標準溶液所繪制的鉀標準曲線較為接近,R2分別高達0.994 4、0.999 7、0.999 8。采用H2O2消煮后所測得的K+濃度最高,2株解鉀菌的解鉀效率分別達到101.1%、125.1%,與其他處理組之間有顯著性差異。用H2O2溶液處理所得到的解鉀率更能真實反映解鉀菌的解鉀作用。 Using Mesorhizobium sp.S-15 and Paenibacillus sp.S-17 as experimental strains.After cell disruption,NH4OAc extraction,H2O2 solution digestion or without any pre-treatment,K+ content in the fermentation broth of potassium solubilizing bacteria was determined with flame photometer.The efficiency of K solubilizing by potassium solubilizing bacteria in the culture medium was calculated.The results showed that the standard curves of the three kinds of K series of standard solutions were similar with the R2 values of 0.994 4,0.999 7 and 0.999 8,respectively.After digested by H2O2 solution,concentration of K+ was detected.The efficiency of two strains was 101.1% and 125.1%,significantly higher than that of other groups.The efficiency of potassium solubilizing detected after H2O2 digestion well reflected potassium solubilization of bacteria. 解鉀菌; 解鉀效率; 鉀離子; 生物鉀肥; 前處理方法; 檢測方法 potassium solubilizing bacteria; efficiency of potassium solubilizing; K+ ; bio-potassium fertilizer; pre-treatment methods; detection method 王珣玨,黃巧云,蔡鵬,陳雯莉 /hznydxzr/article/abstract/20160113
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