1000-2421 article 蘿卜蚜端粒酶TERT基因的鑒定與表達分析 Identification and expression of the TERT in Lipaphis erysimi (Kaltenbach) 為探究端粒酶逆轉錄酶TERT (Telomerase Reverse Transcriptase)基因在昆蟲生長發育過程中的作用機制,本研究以蘿卜蚜Lipaphis erysimi (Kaltenbach)為研究對象克隆了其TERT基因并分析了時空和時間表達情況。結果發現,蘿卜蚜TERT基因的CDS區,長2 658 bp,能夠編碼885個氨基酸,其二級結構含41個α-螺旋,60個卷曲螺旋。系統進化分析表明,蘿卜蚜TERT基因與麥雙尾蚜Diuraphis noxia關系最近,與桃蚜Myzus persicae、豌豆蚜Acyrthosiphon pisum同源性較高。實時熒光定量PCR顯示,TERT基因在蘿卜蚜生長發育過程中的各個齡期及組織中均有表達,且成蟲TERT基因的相對表達量依次高于1齡、2齡、3齡、4齡若蟲;TERT基因在蘿卜蚜腹部表達量最高,頭部次之,胸部表達量最低,但均低于整蟲的表達量。因此TERT基因在蘿卜蚜的生長發育過程中起重要作用,為進一步研究TERT基因在昆蟲中的生理功能奠定了基礎。 Telomerase has the function of maintaining telomere length, and its catalytic subunit TERT (Telomerase Reverse Transcriptase) is tightly regulated in higher organisms such as mammals, and is usually not expressed in somatic cells. However, in lower eukaryotes such as invertebrates, TERT is often expressed in somatic cells, but the physiological function is not clear. In order to clarify the physiological function of TERT, the TERT gene of Lipaphis erysimi (Kaltenbach) was cloned and analyzed for its spatial and temporal expression. The results showed that the CDS region of the radish TERT gene was 2658 bp and capable of encoding 885 amino acids. Its secondary structure contained 41 α-helices and 60 coiled coils. Phylogenetic analysis indicated that the Lipaphis erysimi TERT gene has the closest relationship with Diuraphis noxia, and has high homology with Myzus persicae and Acyrthosiphon pisum, but has low homology with other insects. Real-time quantitative PCR showed that the TERT gene was expressed in all ages and tissues during the growth and development of Lipaphis erysimi,and the relative expression of adult TERT gene was higher than that of 1st, 2nd, 3rd and 4th nymphs. The expression of TERT gene in the abdomen of the Lipaphis erysimi was the highest, followed by the head, and the lowest in the chest, but lower than the expression of the whole worm. This study laid the foundation for further study of the physiological functions of TERT in insects. 端粒酶;TERT;蘿卜蚜;基因克隆;表達分析 telomerase; telomerase reverse transcriptase; Lipaphis erysimi; gene clone; expression pattern analysis 張鑫澤 zhangxinze 華中農業大學 /hznydxzr/article/abstract/L201909005
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